Elucidating the behavior of an enzyme

25-Jul-2019 18:03

Given the structural diversity of compounds produced using these two simple building blocks, there has been longstanding interest in engineering the incorporation of alternative extender units.

We have been investigating the mechanism of fluorinated monomer insertion by three of the six different modules of the PKS involved in erythromycin biosynthesis (6-deoxyerythronolide B synthase, DEBS) to begin understanding the contribution of different steps, such as enzyme acylation, transacylation, C–C bond formation, and chain transfer, to the overall selectivity and efficiency of this process.

The protein is shown as a ribbon diagram, with alpha helices in red, beta sheets in yellow and loops in blue. Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes.

Background: The molecular understanding of factors that limit enzymatic hydrolysis of cellulose remains incomplete.

Results: Pre-steady-state analysis of cellulolytic activity provides rate constants for basic steps of the overall reaction.

By increasing the throughput of fluorinated extender units, we can produce multiply fluorinated polyketide products by chemoenzymatic synthesis and target the production of complex structures.

Polyketides are a large family of bioactive natural products synthesized by polyketide synthase (PKS) enzyme complexes predominantly from acetate and propionate.

The protein is shown as a ribbon diagram, with alpha helices in red, beta sheets in yellow and loops in blue. Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes.

Background: The molecular understanding of factors that limit enzymatic hydrolysis of cellulose remains incomplete.

Results: Pre-steady-state analysis of cellulolytic activity provides rate constants for basic steps of the overall reaction.

By increasing the throughput of fluorinated extender units, we can produce multiply fluorinated polyketide products by chemoenzymatic synthesis and target the production of complex structures.

Polyketides are a large family of bioactive natural products synthesized by polyketide synthase (PKS) enzyme complexes predominantly from acetate and propionate.

This is the first article that clearly demonstrates the gene dosage effects on the amount of the glycosyltransferase protein, its activity, and the amounts of carbohydrate products enzyme assays, using chimera enzymes, demonstrated that the single amino acid substitutions in the catalytic domains of the le1 and le2 enzymes, Gly170 to Ser and Ile367 to Lys, respectively, cause inactivation of the mutant enzymes (Nishihara , 1994).